{"id":62894,"date":"2026-09-12T09:12:44","date_gmt":"2026-09-12T09:12:44","guid":{"rendered":"https:\/\/stagvape.com\/tobacco-plants-nicotine-biosynthesis-pathway"},"modified":"2026-09-13T09:39:50","modified_gmt":"2026-09-13T09:39:50","slug":"tobacco-plants-nicotine-biosynthesis-pathway","status":"publish","type":"post","link":"https:\/\/stagvape.com\/cs\/studie-o-vapingu\/tobacco-plants-nicotine-biosynthesis-pathway","title":{"rendered":"V\u011bdci zjistili, jak tab\u00e1kov\u00e9 rostliny vytv\u00e1\u0159ej\u00ed nikotin, aby umo\u017enili p\u011bstov\u00e1n\u00ed tab\u00e1ku s n\u00edzk\u00fdm obsahem nikotinu"},"content":{"rendered":"\n<p class=\"wp-block-paragraph\">Biochemici z Univerzity v Yorku ve spolupr\u00e1ci s mezin\u00e1rodn\u00edmi partnery dokon\u010dili popis enzymatick\u00e9 dr\u00e1hy, kterou tab\u00e1kov\u00e9 rostliny pou\u017e\u00edvaj\u00ed k sestaven\u00ed nikotinu z jednoduch\u00fdch chemick\u00fdch stavebn\u00edch blok\u016f. Tento objev \u0159e\u0161\u00ed biologickou z\u00e1hadu, kter\u00e1 p\u0159etrv\u00e1vala od doby, kdy chemici v roce 1828 poprv\u00e9 extrahovali nikotin z tab\u00e1kov\u00fdch list\u016f.<\/p>\n\n<p class=\"wp-block-paragraph\">Zji\u0161t\u011bn\u00ed objas\u0148uj\u00ed genetick\u00e9 a biochemick\u00e9 mechanismy odpov\u011bdn\u00e9 za produkci n\u00e1vykov\u00e9ho alkaloidu. Krom\u011b dopad\u016f na zem\u011bd\u011blsk\u00e9 p\u011bstov\u00e1n\u00ed tab\u00e1ku poskytuje v\u00fdzkum molekul\u00e1rn\u00ed n\u00e1stroje k odstran\u011bn\u00ed nikotinu z druh\u016f tab\u00e1ku vyu\u017e\u00edvan\u00fdch jako v\u00fdrobn\u00ed platformy pro l\u00e9\u010diva, vakc\u00edny a diagnostick\u00e9 proteiny.<\/p>\n\n<p class=\"wp-block-paragraph\">\u201eJe to v\u00fdznamn\u00fd okam\u017eik pro rostlinnou v\u011bdu a biochemii, proto\u017ee nyn\u00ed m\u00e1me odpov\u011b\u010f, kterou jsme hledali v\u00edce ne\u017e 200 let!\u201c uvedl Dr. Benjamin Lichman z Centra pro nov\u00e9 zem\u011bd\u011blsk\u00e9 produkty na Univerzit\u011b v Yorku.<\/p>\n\n<h2 class=\"wp-block-heading\">Dvousetlet\u00e9 hled\u00e1n\u00ed biosynt\u00e9zy nikotinu<\/h2>\n\n<p class=\"wp-block-paragraph\">Nikotin v p\u0159\u00edrod\u011b prim\u00e1rn\u011b funguje jako alkaloidn\u00ed obrann\u00fd mechanismus. Tab\u00e1kov\u00e9 rostliny vytv\u00e1\u0159ej\u00ed tuto slou\u010deninu v ko\u0159enech a transportuj\u00ed ji do list\u016f, aby odradily b\u00fdlo\u017erav\u00fd hmyz a sp\u00e1saj\u00edc\u00ed zv\u00ed\u0159ata. P\u0159i konzumaci \u010dlov\u011bkem se molekula p\u0159\u00edmo v\u00e1\u017ee na nikotinov\u00e9 acetylcholinov\u00e9 receptory v nervov\u00e9m syst\u00e9mu, p\u016fsob\u00ed jako stimulant a vyvol\u00e1v\u00e1 fyzickou z\u00e1vislost.<\/p>\n\n<p class=\"wp-block-paragraph\">Po desetilet\u00ed v\u011bdci znali z\u00e1kladn\u00ed chemick\u00e9 prekurzory pot\u0159ebn\u00e9 pro tvorbu nikotinu. Strukturn\u00ed anal\u00fdzy potvrdily, \u017ee se molekula skl\u00e1d\u00e1 ze dvou odli\u0161n\u00fdch heterocyklick\u00fdch kruh\u016f spojen\u00fdch jedinou vazbou uhl\u00edk\u2013uhl\u00edk:<\/p>\n\n<ul class=\"wp-block-list\">\n<li><strong>Pyridinov\u00fd kruh:<\/strong>\u00a0Odvozen\u00fd od kyseliny nikotinov\u00e9 (formy vitaminu B3).<\/li>\n\n\n\n<li><strong>Pyrrolidinov\u00fd kruh:<\/strong>\u00a0Vznik\u00e1 z kationtu N-methylpyrrolinia odvozen\u00e9ho od aminokyseliny.<\/li>\n<\/ul>\n\n<p class=\"wp-block-paragraph\">Navzdory znalosti v\u00fdchoz\u00edch l\u00e1tek v\u011bdci nedok\u00e1zali identifikovat katalytick\u00fd mechanismus odpov\u011bdn\u00fd za spojen\u00ed obou kruh\u016f. Pokusy o pozorov\u00e1n\u00ed p\u0159\u00edm\u00e9 kondenzace t\u011bchto molekul v laboratorn\u00edm prost\u0159ed\u00ed opakovan\u011b selh\u00e1valy, proto\u017ee kyselina nikotinov\u00e1 z\u016fst\u00e1v\u00e1 za standardn\u00edch biologick\u00fdch podm\u00ednek chemicky stabiln\u00ed. Bez aktiva\u010dn\u00edho kroku z\u016fst\u00e1vala energetick\u00e1 bari\u00e9ra pro spojen\u00ed kruh\u016f p\u0159\u00edli\u0161 vysok\u00e1, ne\u017e aby ji zn\u00e1m\u00e9 rostlinn\u00e9 enzymy dok\u00e1zaly p\u0159ekonat.<\/p>\n\n<figure class=\"wp-block-image size-large\"><img fetchpriority=\"high\" decoding=\"async\" width=\"1024\" height=\"376\" src=\"https:\/\/stagvape.com\/wp-content\/uploads\/2026\/09\/image-2-1024x376.png\" alt=\"Jak tab\u00e1kov\u00e9 rostliny vytv\u00e1\u0159ej\u00ed nikotin\" class=\"wp-image-62515\" title=\"\" srcset=\"\/\/stagvape.com\/wp-content\/uploads\/2026\/09\/image-2-1024x376.png 1024w, \/\/stagvape.com\/wp-content\/uploads\/2026\/09\/image-2-300x110.png 300w, \/\/stagvape.com\/wp-content\/uploads\/2026\/09\/image-2-768x282.png 768w, \/\/stagvape.com\/wp-content\/uploads\/2026\/09\/image-2-1536x564.png 1536w, \/\/stagvape.com\/wp-content\/uploads\/2026\/09\/image-2.png 1920w\" sizes=\"(max-width: 1024px) 100vw, 1024px\" \/><\/figure>\n\n<h2 class=\"wp-block-heading\">Mechanismus: skryt\u00e1 aktiva\u010dn\u00ed glukosylace<\/h2>\n\n<p class=\"wp-block-paragraph\">T\u00fdm z Univerzity v Yorku zjistil, \u017ee tab\u00e1kov\u00e1 rostlina tuto chemickou bari\u00e9ru obch\u00e1z\u00ed vyu\u017eit\u00edm ne\u010dekan\u00e9ho meziproduktu: gluk\u00f3zy. Rostlina do\u010dasn\u011b nav\u00e1\u017ee molekulu cukru gluk\u00f3zy na kyselinu nikotinovou, \u010d\u00edm\u017e slou\u010deninu destabilizuje a sn\u00ed\u017e\u00ed aktiva\u010dn\u00ed energii pot\u0159ebnou pro n\u00e1sledn\u00e9 enzymatick\u00e9 reakce.<\/p>\n\n<p class=\"wp-block-paragraph\">Tato biologick\u00e1 strategie, ozna\u010dovan\u00e1 jako <strong>skryt\u00e1 aktiva\u010dn\u00ed glukosylace<\/strong>, funguje jako do\u010dasn\u00fd molekul\u00e1rn\u00ed \u00fachyt. Jakmile gluk\u00f3za aktivuje prekurzor, dal\u0161\u00ed enzymy spoj\u00ed kruh s N-methylpyrroliniem. Ihned po vytvo\u0159en\u00ed strukturn\u00ed vazby jin\u00fd enzym od\u0161t\u011bp\u00ed cukr od komplexu a zanech\u00e1 hotovou molekulu nikotinu.<\/p>\n\n<p class=\"wp-block-paragraph\">Proto\u017ee se molekula gluk\u00f3zy odpoj\u00ed d\u0159\u00edve, ne\u017e kone\u010dn\u00fd produkt opust\u00ed enzymatick\u00fd komplex, nezanech\u00e1v\u00e1 v hotov\u00e9m alkaloidu \u017e\u00e1dnou chemickou stopu. Tato p\u0159echodn\u00e1 povaha vysv\u011btluje, pro\u010d generace analytick\u00fdch chemik\u016f nedok\u00e1zaly izolovat mezistupn\u011b t\u00e9to dr\u00e1hy.<\/p>\n\n<p class=\"wp-block-paragraph\">\u201eZ\u00e1hada, jak tab\u00e1kov\u00e9 rostliny produkuj\u00ed nikotin, existuje od konce 20. let 19. stolet\u00ed, kdy byl nikotin z rostlin poprv\u00e9 extrahov\u00e1n,\u201c \u0159ekl Lichman. \u201eS t\u011bmito nov\u00fdmi poznatky m\u016f\u017eeme odstranit nebo p\u0159eorientovat nikotin, kter\u00fd rostlina p\u0159irozen\u011b produkuje, a vytvo\u0159it lep\u0161\u00ed biotechnologick\u00e9 n\u00e1stroje. Do budoucna je zde tak\u00e9 vzru\u0161uj\u00edc\u00ed potenci\u00e1l p\u0159izp\u016fsobit syst\u00e9m tvorby nikotinu v tab\u00e1ku k v\u00fdrob\u011b u\u017eite\u010dn\u00fdch farmaceutick\u00fdch slou\u010denin.\u201c<\/p>\n\n<h2 class=\"wp-block-heading\">Kask\u00e1da \u010dty\u0159 enzym\u016f<\/h2>\n\n<p class=\"wp-block-paragraph\">Aby ov\u011b\u0159il sekvenci reakc\u00ed, v\u00fdzkumn\u00fd t\u00fdm izoloval a purifikoval \u010dty\u0159i odli\u0161n\u00e9 enzymy z tab\u00e1kov\u00fdch pletiv a rekonstituoval celou metabolickou dr\u00e1hu mimo \u017eivou bu\u0148ku.<\/p>\n\n<figure class=\"wp-block-table\"><table class=\"has-fixed-layout\"><thead><tr><th class=\"has-text-align-left\" data-align=\"left\">Enzym<\/th><th class=\"has-text-align-left\" data-align=\"left\">Biochemick\u00e1 funkce<\/th><th class=\"has-text-align-left\" data-align=\"left\">\u00daloha v dr\u00e1ze<\/th><\/tr><\/thead><tbody><tr><td><strong>NaGT<\/strong><\/td><td>Glukosyltransfer\u00e1za<\/td><td>P\u0159ipojuje gluk\u00f3zu ke kyselin\u011b nikotinov\u00e9 a vytv\u00e1\u0159\u00ed aktivovan\u00fd meziprodukt.<\/td><\/tr><tr><td><strong>NaGR<\/strong><\/td><td>Redukt\u00e1za<\/td><td>P\u0159en\u00e1\u0161\u00ed atomy vod\u00edku za \u00fa\u010delem redukce aktivovan\u00e9 molekuly prekurzoru.<\/td><\/tr><tr><td><strong>NicGS<\/strong><\/td><td>Synt\u00e1za spojov\u00e1n\u00ed<\/td><td>Zaji\u0161\u0165uje stereochemickou kontrolu a produkuje biologicky aktivn\u00ed (S)-nikotin.<\/td><\/tr><tr><td><strong>NicGH<\/strong><\/td><td>Glukosid\u00e1za \/ hydrol\u00e1za<\/td><td>Hydrolyzuje a odstra\u0148uje do\u010dasn\u011b p\u0159ipojenou gluk\u00f3zu, \u010d\u00edm\u017e vznik\u00e1 \u010dist\u00fd nikotin.<\/td><\/tr><\/tbody><\/table><\/figure>\n\n<p class=\"wp-block-paragraph\">Pomoc\u00ed rentgenov\u00e9 krystalografie s vysok\u00fdm rozli\u0161en\u00edm hlavn\u00ed autor Benjamin Schwabe ur\u010dil trojrozm\u011brn\u00e9 struktury NaGR a NicGS na atomov\u00e9m rozli\u0161en\u00ed. Struktur\u00e1ln\u00ed modely uk\u00e1zaly, \u017ee NicGS fyzicky orientuje reak\u010dn\u00ed meziprodukty ve sv\u00e9 vazebn\u00e9 kapse tak, aby \u0159\u00eddila prostorovou konfiguraci v\u00fdsledn\u00e9 vazby.<\/p>\n\n<p class=\"wp-block-paragraph\">Tato struktur\u00e1ln\u00ed kontrola je biologicky rozhoduj\u00edc\u00ed. Biologick\u00e9 molekuly \u010dasto existuj\u00ed jako chir\u00e1ln\u00ed enantiomery \u2014 zrcadlov\u00e9 varianty stejn\u00e9ho chemick\u00e9ho vzorce. Tab\u00e1kov\u00e9 rostliny produkuj\u00ed t\u00e9m\u011b\u0159 v\u00fdhradn\u011b (S)-nikotin, optick\u00fd izomer, kter\u00fd se \u00fa\u010dinn\u011b v\u00e1\u017ee na neurologick\u00e9 receptory savc\u016f. Kdy\u017e v\u00fdzkumn\u00edci testovali dr\u00e1hu bez NicGS, syst\u00e9m vytvo\u0159il nekontrolovanou racemickou sm\u011bs levoto\u010div\u00fdch i pravoto\u010div\u00fdch izomer\u016f, co\u017e prok\u00e1zalo, \u017ee NicGS ur\u010duje stereochemickou p\u0159esnost p\u0159\u00edrodn\u00edho tab\u00e1ku.<\/p>\n\n<h2 class=\"wp-block-heading\">Ov\u011b\u0159en\u00ed v \u017eiv\u00fdch rostlinn\u00fdch syst\u00e9mech<\/h2>\n\n<p class=\"wp-block-paragraph\">Po bezbun\u011b\u010dn\u00e9 laboratorn\u00ed synt\u00e9ze v\u00fdzkumn\u00edci ov\u011b\u0159ili enzymatickou dr\u00e1hu v \u017eiv\u00e9 rostlinn\u00e9 tk\u00e1ni. Geny k\u00f3duj\u00edc\u00ed \u010dty\u0159i enzymy vlo\u017eili do <em>Nicotiana benthamiana<\/em>, divok\u00e9ho australsk\u00e9ho p\u0159\u00edbuzn\u00e9ho tab\u00e1ku, kter\u00fd se \u0161iroce pou\u017e\u00edv\u00e1 v zem\u011bd\u011blsk\u00e9m a farmaceutick\u00e9m v\u00fdzkumu.<\/p>\n\n<p class=\"wp-block-paragraph\">Upraven\u00e9 rostliny produkovaly zna\u010den\u00e9 molekuly nikotinu, kter\u00e9 odpov\u00eddaly p\u0159irozen\u00fdm profil\u016fm rostlin. V\u00fdzkumn\u00edci d\u00e1le ov\u011b\u0159ili ka\u017ed\u00fd krok vy\u0159azen\u00edm jednotliv\u00fdch gen\u016f v kask\u00e1d\u011b:<\/p>\n\n<ul class=\"wp-block-list\">\n<li>Potla\u010den\u00ed <strong>NicGH<\/strong> zp\u016fsobilo, \u017ee rostliny hromadily stabiln\u00ed prekurzory nikotinu nav\u00e1zan\u00e9 na gluk\u00f3zu, ani\u017e by produkovaly voln\u00fd nikotin.<\/li>\n\n\n\n<li>Vynech\u00e1n\u00ed <strong>NicGS<\/strong> naru\u0161ilo stereospecificitu dr\u00e1hy, \u010d\u00edm\u017e potvrdilo jej\u00ed regula\u010dn\u00ed \u00falohu v \u017eiv\u00e9m bun\u011b\u010dn\u00e9m prost\u0159ed\u00ed.<\/li>\n<\/ul>\n\n<h2 class=\"wp-block-heading\">Zlep\u0161ov\u00e1n\u00ed tab\u00e1kov\u00fdch platforem pro molekul\u00e1rn\u00ed zem\u011bd\u011blstv\u00ed<\/h2>\n\n<p class=\"wp-block-paragraph\">Identifikace t\u011bchto \u010dty\u0159 enzym\u016f m\u00e1 p\u0159\u00edm\u00e9 vyu\u017eit\u00ed v modern\u00ed biov\u00fdrob\u011b. A\u010dkoli je komer\u010dn\u00ed tab\u00e1k st\u00e1le spojov\u00e1n p\u0159edev\u0161\u00edm s cigaretami, rostlinn\u00ed biologov\u00e9 \u010dasto vyu\u017e\u00edvaj\u00ed druhy tab\u00e1ku pro molekul\u00e1rn\u00ed zem\u011bd\u011blstv\u00ed \u2014 rychle rostouc\u00ed rostlinn\u00e1 pletiva pou\u017e\u00edvaj\u00ed jako bioreaktory k synt\u00e9ze l\u00e9\u010debn\u00fdch protein\u016f, monoklon\u00e1ln\u00edch protil\u00e1tek a vakcina\u010dn\u00edch antigen\u016f.<\/p>\n\n<p class=\"wp-block-paragraph\"><em>Nicotiana benthamiana<\/em> je v molekul\u00e1rn\u00edm zem\u011bd\u011blstv\u00ed preferov\u00e1na, proto\u017ee b\u011bhem n\u011bkolika t\u00fddn\u016f poskytuje velk\u00e9 mno\u017estv\u00ed biomasy a snadno p\u0159ij\u00edm\u00e1 vektory pro p\u0159echodnou expresi. P\u0159irozen\u00e1 produkce nikotinu v\u0161ak p\u0159edstavuje trvalou v\u00fdrobn\u00ed p\u0159ek\u00e1\u017eku. Alkaloid je pro \u010dlov\u011bka v koncentrac\u00edch pou\u017e\u00edvan\u00fdch ve farmaceutick\u00e9 v\u00fdrob\u011b toxick\u00fd a spole\u010dn\u011b se \u010dist\u00ed s c\u00edlov\u00fdmi proteiny, co\u017e vy\u017eaduje n\u00e1kladn\u00e9 v\u00edcestup\u0148ov\u00e9 filtra\u010dn\u00ed procesy k izolaci terapeutick\u00fdch molekul.<\/p>\n\n<p class=\"wp-block-paragraph\">\u201eTab\u00e1kov\u00e9 rostliny lze v biotechnologi\u00edch vyu\u017e\u00edvat jako platformy pro v\u00fdrobu vakc\u00edn nebo jin\u00fdch farmaceutick\u00fdch produkt\u016f, jejich vyu\u017eit\u00ed v\u0161ak komplikuje p\u0159\u00edtomnost nikotinu, kter\u00fd produkty kontaminuje a vy\u017eaduje zpracov\u00e1n\u00ed k jeho odstran\u011bn\u00ed,\u201c vysv\u011btlil Lichman.<\/p>\n\n<p class=\"wp-block-paragraph\">Pou\u017eit\u00edm c\u00edlen\u00fdch n\u00e1stroj\u016f pro \u00fapravu gen\u016f, jako je CRISPR, k deaktivaci gen\u016f k\u00f3duj\u00edc\u00edch NaGT nebo NicGS mohou zem\u011bd\u011bl\u0161t\u00ed v\u011bdci vy\u0161lechtit tab\u00e1kov\u00e9 linie bez nikotinu. Tyto rostliny si zachov\u00e1vaj\u00ed rychl\u00fd vegetativn\u00ed r\u016fst a schopnost synt\u00e9zy protein\u016f, p\u0159i\u010dem\u017e zcela eliminuj\u00ed kontaminaci alkaloidy a sni\u017euj\u00ed v\u00fdrobn\u00ed n\u00e1klady l\u00e9\u010div z\u00edsk\u00e1van\u00fdch z rostlin.<\/p>\n\n<h2 class=\"wp-block-heading\">V\u00fdvoj nov\u00fdch farmaceutick\u00fdch alkaloid\u016f<\/h2>\n\n<p class=\"wp-block-paragraph\">Objev rovn\u011b\u017e vytv\u00e1\u0159\u00ed enzymatick\u00fd r\u00e1mec pro synt\u00e9zu nen\u00e1vykov\u00fdch l\u00e9\u010div\u00fdch slou\u010denin. Modul\u00e1rn\u00ed povaha syst\u00e9mu \u010dty\u0159 enzym\u016f umo\u017e\u0148uje v\u00fdzkumn\u00edk\u016fm dod\u00e1vat do kask\u00e1dy alternativn\u00ed prekurzorov\u00e9 molekuly.<\/p>\n\n<p class=\"wp-block-paragraph\">V ov\u011b\u0159ovac\u00edch experimentech v\u00fdzkumn\u00fd t\u00fdm prok\u00e1zal, \u017ee nahrazen\u00ed prekurzorov\u00fdmi analogy umo\u017enilo enzym\u016fm vytv\u00e1\u0159et p\u0159\u00edbuzn\u00e9 alkaloidy, v\u010detn\u011b nornikotinu a anabasinu. Vzhledem k tomu, \u017ee mnoho rostlinn\u00fdch alkaloid\u016f slou\u017e\u00ed jako strukturn\u00ed z\u00e1klad pro l\u00e9\u010diva p\u016fsob\u00edc\u00ed na centr\u00e1ln\u00ed nervov\u00fd syst\u00e9m, terapie bolesti a insekticidy, nab\u00edz\u00ed p\u0159izp\u016fsoben\u00ed tohoto enzymatick\u00e9ho mechanismu \u010dist\u00fd zp\u016fsob v\u00fdroby komplexn\u00edch chemick\u00fdch slou\u010denin bez pr\u016fmyslov\u00e9 synt\u00e9zy p\u0159i vysok\u00fdch teplot\u00e1ch.<\/p>\n\n<p class=\"wp-block-paragraph\">\u201eOtev\u00edr\u00e1 to nov\u00e9 mo\u017enosti, jak tab\u00e1kov\u00e9 rostliny vyu\u017e\u00edt k dobr\u00e9mu: ne v cigaret\u00e1ch, ale pro l\u00e9\u010diva a dal\u0161\u00ed hodnotn\u00e9 produkty,\u201c \u0159ekl Lichman.<\/p>\n\n<ul class=\"wp-block-list\">\n<li>V\u00fdsledky v\u00fdzkumu jsou dostupn\u00e9 online v \u010dasopise <a href=\"https:\/\/www.nature.com\/articles\/s41467-026-72705-0\" target=\"_blank\" rel=\"noopener nofollow\">Nature Communications<\/a>.<\/li>\n<\/ul>\n","protected":false},"excerpt":{"rendered":"<p>V\u00fdzkumn\u00edci z Univerzity v Yorku vy\u0159e\u0161ili 200 let starou z\u00e1hadu a zmapovali enzymy, kter\u00e9 sestavuj\u00ed nikotin, co\u017e umo\u017en\u00ed p\u011bstov\u00e1n\u00ed plodin s n\u00edzk\u00fdm obsahem nikotinu.<\/p>\n","protected":false},"author":1,"featured_media":62895,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"_acf_changed":false,"footnotes":""},"categories":[2038],"tags":[2829],"class_list":["post-62894","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-studie-o-vapingu","tag-nicotine-2"],"acf":[],"_links":{"self":[{"href":"https:\/\/stagvape.com\/cs\/wp-json\/wp\/v2\/posts\/62894","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/stagvape.com\/cs\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/stagvape.com\/cs\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/stagvape.com\/cs\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/stagvape.com\/cs\/wp-json\/wp\/v2\/comments?post=62894"}],"version-history":[{"count":0,"href":"https:\/\/stagvape.com\/cs\/wp-json\/wp\/v2\/posts\/62894\/revisions"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/stagvape.com\/cs\/wp-json\/wp\/v2\/media\/62895"}],"wp:attachment":[{"href":"https:\/\/stagvape.com\/cs\/wp-json\/wp\/v2\/media?parent=62894"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/stagvape.com\/cs\/wp-json\/wp\/v2\/categories?post=62894"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/stagvape.com\/cs\/wp-json\/wp\/v2\/tags?post=62894"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}